News

New mRNA Expression Browser (Beta)

June 7, 2017

The browser (beta version) for the visualization of mRNA expression from CHO-K1 cells is now online. The data is from several published DNA-microarray or RNA-Seq experiments. The tutorial on how to use this browser can be found here.

Coming Soon!

March 10, 2017

Users will be able to visualize CHO cell mRNA expression data either from published DNA-microarray or RNA-Seq experiments via JBrowse. The visualized data will enable users to easily obtain gene expression levels and compare gene expression levels among different conditions.

CHOmine (BETA-Version)

August 19, 2016

CHOmine, the InterMine site for CHO and Chinese hamster, went online on August 11, 2016 at https://chomine.boku.ac.at/. CHOmine collects and integrates publicly available Chinese hamster and CHO data, where different datasets are connected to provide user-friendly access. Currently, users are asked to test the site and to provide feedback (impressions, comments, issues, and suggestions for improvements) to Matthias.Gerstl@acib.at or Nicole.Borth@boku.ac.at.

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CHO-K1 reference map

Click on a quadrant to view proteins.

Experimental Details

CHO-K1 cells were grown in Ham’s F12 medium supplemented with 10% fetal bovine serum.

Samples were separated on a 3-10 linear pH gradient and 12%T polyacrylamide gel.  Gels were stained with SYPRO Ruby and MALDI-TOF/TOF was used to identify spots.

Reference

Electrophoresis (2004) 25, 2545