News

Updated CH assembly and CH/CHO-K1 annotations

May 1, 2019

We are excited to announce that the significantly improved Chinese hamster genome, CH PICR (GCF_00366804.1), along with its 2018 RefSeq annotation is now online. In addition, an updated RefSeq annotation for the CHO-K1 cell line is now available. Both can be searched (gene search and BLAST) and viewed on JBrowse. More information on the updated Chinese hamster genome can be found here.

New mRNA Expression Browser (Beta)

June 7, 2017

The browser (beta version) for the visualization of mRNA expression from CHO-K1 cells is now online. The data is from several published DNA-microarray or RNA-Seq experiments. The tutorial on how to use this browser can be found here.

Coming Soon!

March 10, 2017

Users will be able to visualize CHO cell mRNA expression data either from published DNA-microarray or RNA-Seq experiments via JBrowse. The visualized data will enable users to easily obtain gene expression levels and compare gene expression levels among different conditions.

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CHO-K1 reference map

Click on a quadrant to view proteins.

Experimental Details

CHO-K1 cells were grown in Ham’s F12 medium supplemented with 10% fetal bovine serum.

Samples were separated on a 3-10 linear pH gradient and 12%T polyacrylamide gel.  Gels were stained with SYPRO Ruby and MALDI-TOF/TOF was used to identify spots.

Reference

Electrophoresis (2004) 25, 2545